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 VARIANTS / TOUCHDOWN PCR

 

Selected and organized links about Touchdown PCR generalities and applications.

 

 


Touchdown PCR is another modification of conventional PCR that may result in a reduction of nonspecific amplification. It involves the use of an annealing temperature that is higher than the target optimum in early PCR cycles. The annealing temperature is decreased by 1C every cycle or every second cycle until a specified or 'touchdown' annealing temperature is reached. The touchdown temperature is then used for the remaining number of cycles. This allows for the enrichment of the correct product over any non-specific product.
Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples



'Touchdown' PCR to circumvent spurious priming during gene amplification
File Format: PDF/ Adobe Acrobat
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS.
Nucleic Acids Res. 1991 Jul 25;19(14):4008.

Berglund:Touchdown PCR
Author: OpenWetWare contributors
Publisher: OpenWetWare
 

 

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