AFLP


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VARIANTS / AFLP:AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS

Selected and organized links about AFLP generalities, protocols and applications.

Definition
AFLP-PCR was originally described by Zabeau & Vos in 1993. The procedure of this technique is divided into three steps: 1)Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments. 2)Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences. 3) Electrophoretic separation and amplicons on a gel matrix, followed by visualisation of the band pattern.
Source:
http://taxonomy.zoology.gla.ac.uk/~rdmp1c/teaching/L4/
Evolution/Session5/Mueller.pdf

L i n k s

· AFLP genotyping and fingerprinting
A review about advantages and applications of the AFLP technique.
Ulrich G. Mueller and L. LaReesa Wolfenbarger (1999),Tree.
File Format: PDF/ Adobe Acrobat

· Amplified Fragment Length Polymorphisms and Microsatellites: A phylogenetic perspective
This paper reviews the techniques of AFLP and Simple Sequence Repeats, the basis of the polymorphisms they identify; their advantages and disadvantages; their methods of analysis and their applicability to systematic studies
Julian P. Robinson and Stephen A. Harris (1999)
Chapter 12 in: Which DNA Marker for Which Purpose?

· AFLP®: principles and applications
Describes with text, pictures and animations the different steps of the AFLP method.
Keygene

· AFLP's
Explain the concepts of AFLP technique.
Ulrich Melcher
Molecular Genetics

· Standard List for AFLP® Primer Nomenclature
GrainGenes

/P r o t o c o l s

· AFLP: not only for fingerprinting, but for positional cloning
Mannie Liscum and Paul Oeller
Department of Plant Biology, Carnegie Institution of Washington

· AFLP protocol
Samuel Hazen, Michigan State University

· AFLP protocol
This protocol has been adapted from several sources
Paul G. Wolf , Utah State University

· AFLPs on the ABI 3100
This protocols were developed for doing AFLP’s (or AFLP-MITEs) on an ABI3100 capillary sequencer
Maize Mapping Project

· AFLP
Dr. Michael Havey's Vegetable Genetics Laboratory

· Amplified Fragment Length Polymorphism Protocol
Standard European Working Group on Legionella Infections

· AFLP Technologies
This page should serve as a useful reference tool for other researchers currently using or wishing to use AFLP technology
Liscum Lab, University of Missouri.

- New addition -

Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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