Hot Start PCR


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VARIANTS / HOT START PCR

Selected and organized links about Hot Start PCR.

"Hot-start" PCR is a modification of conventional PCR that reduces non-specific product amplification. In this procedure amplification cannot occur until the reaction temperature is above that where non-specific annealing of primers to targets occurs. This block in amplification is usually accomplished by using a DNA polymerase that is inactive until higher temperatures are reached.
Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples

An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose
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Kaijalainen S, Karhunen PJ, Lalu K, Lindström K.
Nucleic Acids Res. 1993 Jun 25; 21(12): 2959-2960.

PCR hot start using primers with the structure of molecular beacons (hairpin-like structure).
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Kaboev OK, Luchkina LA, Tret’iakov AN, Bahrmand AR.
Nucleic Acids Res. 2000 Nov 1; 28(21): e94.

Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.
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Kermekchiev MB, Tzekov A, Barnes WM.
Nucleic Acids Res. 2003 Nov 1; 31(21): 6139-6147.

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