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L i n k s
· PCR
Primer design and Reaction Optimisation
Molecular Biology Techniques Manual,
Third Edition
Ed Rybicki
Department of Molecular and Cell Biology, University of Cape Town
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General
Notes on Primer Design in PCR
This application note is provided to give rules that should be taken into
account when designing primers for PCR
Vincent R. Prezioso
Eppendorf North America, Inc.
· Design
of Primers for Automated Sequencing
Describes the steps involved in
Design of Primers for automated DNA sequencing and the major
pitfalls to avoid.
DNA Squencing Core / University of Michigan
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Bioinformatic
tools and guideline for PCR primer design
This review summarizes the general guidelines for primer design online.
File Format: PDF/ Adobe Acrobat
Kamel A. Abd-Elsalam (2003), African Journal of
Biotechnology
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A critical
review of PCR primer design algorithms and crosshybridization case study
This paper analyzes the algorithms and parameter weightings of commonly used
primer design programs, such as PRIDE, PRIME+, DOPRIMER, PRIMO, Primer Master,
and MEDUSA.
File Format: PDF/ Adobe Acrobat
F. John Burpo (2001), Biochemistry 218
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Primer design
Describes some points to remember in the
design of primers and degenerate oligonucleotides.
The Board of Regents of the University of
Wisconsin System
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