Selected and organized links about Vectorette PCR  protocols and applications.

Vectorette PCR is a method that enables the amplification
of specific DNA fragments in situations where the sequence
of only one primer is known. Thus, it extends the application
of PCR to stretches of DNA where the sequence information
is only available at one end (Arnold and Hodgson 1991).
 

A Vectorette PCR-based approach for sequencing homologous
regions from different genomes
File Format: PDF/ Adobe Acrobat
First describes the principles and applications of the Vectorette
PCR, and then details protocols for the three steps required for
this method
Hiroshi Akashi Lab
Pennsylvania State University
* * * * *

Vectorette PCR isolation of microsatellite repeat sequences
using anchored dinucleotide repeat primers
N J Lench et al., (1996) Nucleic Acids Res
June 1; 24(11): 2190–2191
File Format: PDF/ Adobe Acrobat
PubMed Central (PMC)
National Institutes of Health (NIH)
* * * * *

A novel, rapid method for the isolation of terminal
sequences from yeast artificial chromosome (YAC) clones
File Format: PDF/ Adobe Acrobat
J.Riley et al.,(1990) Nucleic Acids Res 18:2887-90
PubMed Central (PMC)
National Institutes of Health (NIH)
* * * * *

PROTOCOLS


Vectorette End Rescue of Bacterial Clones
File Format: PDF/ Adobe Acrobat
Stéphanie Le Hellard
Medical Genetics
University of Edinburgh
 

Vectorette PCR of mTn-3xHA transposons
Text modified and expanded from that found
at Vectorette PCR of Yeast DNA
Yale genome Analysis Center

 

Vectorette PCR of Yeast DNA
Botstein Lab
Carl Friddle
Stanford University


 

Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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