PCR (Polymerase
Chain Reaction)
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The polymerase chain reaction is a test tube system for DNA replication
that allows a "target" DNA sequence to be selectively amplified, or
enriched, several million-fold in just a few hours
Definition from:
http://www.accessexcellence.org/RC/CT/polymerase_chain_reaction.html
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PCR is a technique for amplifying a specific region of DNA, defined by a
set of two "primers" at which DNA synthesis is initiated by a thermostable
DNA polymerase. Usually, at least a million-fold increase of a specific
section of a DNA molecule can be realized and the PCR product can be
detected by gel electrophoresis. The regions amplified are usually between
150-3,000 base pairs in length.
Definition from:
http://vm.cfsan.fda.gov/~frf/rflp.html
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A technique
for making many copies of a specific DNA sequence. The reaction is
initiated using a pair of short primer sequences which match the ends of
the sequence to be copied. Thereafter, each cycle of the reaction copies
the sequence between the primers. Primers can bind to the copies as well
as the original sequence, so the total number of copies increases
exponentially with time.
Definition from:
Glossary of Biotechnology Terms
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A polymerase chain reaction, or PCR, allows a molecular biologist to
create multiple copies of DNA without using a living organism for
synthesis
Definition from:
ISCID
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A technique for copying the complementary strands
of a target DNA molecule simultaneously for a series of cycles until the
desired amount is obtained
Definition from:
http://ord.aspensys.com/asp/resources/glossary_n-r.asp#P
