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Insertion
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A type of mutation in
which one or more nucleotides is inserted into a DNA sequence. Small
insertions within a gene can alter the reading frame, and thus the amino
acid sequence of the encoded protein.
Definition from:
Mouse Genome Informatics
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Addition of a DNA element into a chromosome
Definition from:
Hypermedia Glossary Of Genetic Terms
In situ PCR
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In situ PCR is a method in which the polymerase chain reaction actually
takes place in the cell on a slide, and the product can be visualized in
the same way as in traditional in situ hybridization.
Definition from:
Molecular
Diagnostic Approach to Non-Hodgkin’s Lymphoma
Daniel A. Arber, 2000
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In situ PCR is a powerful technique in which gene fragments are
amplified within tissue sections.
Definition from:
Diagnostic value of in situ polymerase chain reaction in
leprosy
Dayal
et al., 2005
Intercalating dyes (SYBR Green and similar)
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Real
Time Amplification System.
Despite being the most economical option for detection and quantifying
real time amplified products, intercalating dyes (i.e. SYBR Green), bind
to any double-stranded DNA present in the reaction, including primer-dimers
and other nonspecific reaction products. This requires to take specific
precautions. Therefore most of the protocols for kits based upon this
technology include a melting curve analysis, intended to identify the
amplified product that has been detected.
Definition from:
http://www.biotools.net/eng/tecnica/t33.htm
Intron
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Part of a gene whose sequence is transcribed but not present in a mature
mRNA after splicing.
Definition from:
Mouse Genome Informatics
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DNA sequence that interrupts the protein-coding sequence of a gene; an
intron is transcribed into RNA but is cut out of the message before it is
translated into protein
Definition from:
Genome Glossary
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The DNA base sequence interrupting the protein-coding sequences of a gene;
intron sequences are transcribed into RNA but are cut out of the message
before it is translated into protein. ...
Definition from:
Hypermedia Glossary Of Genetic Terms
Inversion
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A type of mutation in
which a length of DNA is broken in two positions and repaired in such a
way that the medial segment is now present in reverse order. Inversions
range in size from those large enough to be visible cytogenetically to
those involving only a few base pairs.
Definition from:
Mouse Genome Informatics
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The term introduced
by Sturtevant (1926) for the reversal of the linear sequence of the genes
in a segment of a chromosome. In an inversion heterozygote one of the
chromosomes contributed by one parent has an inverted segment while the
homologous chromosome contributed by the other parent has the normal gene
sequence
Definition from:
Hypermedia Glossary Of Genetic Terms
IPCR ( Inverse PCR )
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Variation of PCR that makes the amplification of DNA segments of
unknown sequence that flank DNA segments of known sequence possible; in
brief, total DNA is digested to completion and fragments ligated under
conditions that favour circularization of fragments; pair of PCR primers,
designed from known sequence, are used to prime PCR from opposite strands
resulting in amplification of fragment of unknown sequence.
Definition from:
Glossary of Biochemistry and Molecular Biology
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Inverse PCR is a method used to allow PCR when only one internal
sequence is known. This is especially useful in identifying flanking
sequences to various genomic inserts. This involves a series of digestions
and self ligation before cutting by an endonuclease, resulting in known
sequences at either end of the unknown sequence.
Definition from:
Polymerase Chain
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