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CAPS
(Cleaved Amplified Polymorphic Sequence) See PCR-RFLP
cDNA
(complementary
DNA)
-
DNA
transcribed from an RNA molecule by an enzyme called reverse transcriptase.
Definition
from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf
-
A
DNA sequence which was produced from mRNA by reverse transcription. A cDNA
is so-called because its sequence is the complement of the original mRNA
sequence. However, when double-stranded cDNA is synthesized, it contains
both the original sequence and its complement.
http://www.cs.wustl.edu/~jbuhler/research/array/glossary.html
cDNA library
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A collection of cDNA's, each of which has
been inserted in a DNA vector (e.g. a circular DNA plasmid) and replicated
in a bacterium such as E. coli. The bacteria maintain a ready pool of the
cDNA's and can be cultured to make copies of the library for many
experiments. A population of bacteria containing a single inserted cDNA is
called a clone. To extract a cDNA from the library, the insert from the
corresponding clone can be amplified using PCR primed off the vector
sequences connected to the ends of the cDNA
Definition
from:
http://www.cs.wustl.edu/~jbuhler/research/array/glossary.html
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A pool
of complementary DNA clones produced by cDNA cloning of total messenger
RNA from a single source (cell type, tissue, embryo).
Definition
from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-C/cDNA_library.html
Cloning
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Using specialized DNA technology to produce multiple, exact copies of a
single gene or other segment of DNA to obtain enough material for further
study. ...
Definition
from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-C/cloning.html
-
In
molecular biology: the process of using DNA manipulation procedures to
produce multiple copies of a single gene or DNA segment
Definition
from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf
Cloning vector
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DNA
molecule originating from a virus, a plasmid, or the cell of a higher
organism into which another DNA fragment of appropriate size can be
integrated without loss of the vectors capacity for self- replication;
vectors introduce foreign DNA into host cells, where it can be reproduced
in large quantities. Examples are plasmids, cosmids, and yeast artificial
chromosomes; vectors are often recombinant molecules containing DNA
sequences from several sources.
Definition
from:
http://linkage.rockefeller.edu/wli/glossary/genetics.html
Colony PCR
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Colony PCR is can be used after a transformation to screen colonies for
the desired plasmid. Primers are used which generate a PCR product of
known size. Thus, any colonies which give rise to an amplification product
of the expected size are likely to contain the correct DNA sequence
Definition
from:
OpenWetWare contributors, "Colony PCR," OpenWetWare, ,
http://openwetware.org/index.php?title=Colony_PCR&oldid=11695
(accessed August 26, 2006).
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Use of a bacterial colony for direct polymerase chain reaction to amplify
a specific nucleotide sequence.
Definition
from:
http://www.sci.sdsu.edu/~smaloy/Glossary/C.html
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