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AFLP
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(Amplified Fragment Length Polymorphism)
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Amplified fragment length polymorphism
(AFLP) is a PCR-based technique that involves restriction of genomic DNA
followed by ligation of adaptors to the fragments generated and selective
PCR amplification of a subset of these fragments. The amplified fragments
are separated on a sequencing gel and visualized by autoradiography or
fluorescent sequencing equipment.
Definition from:
A format for
databasing and comparison of AFLP fingerprint profiles
Yan Hong and Aaron Chuah,
2003
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AFLP-PCR
was originally described by Zabeau & Vos in 1993. The procedure of
this technique is divided into three steps: 1)Digestion
of total cellular DNA with one or more restriction enzymes and ligation of
restriction half-site specific adaptors to all restriction fragments.
2)Selective amplification of some of these
fragments with two PCR primers that have corresponding adaptor and
restriction site specific sequences. 3)
Electrophoretic separation and amplicons on a gel matrix, followed by
visualisation of the band pattern.
Definition from:
http://insilico.ehu.es/AFLP/info.html
Alu-PCR
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PCR using a primer that anneals to Alu repeats to amplify DNA located
between two oppositely oriented Alu sequences. Used as a method of
obtaining a fingerprint of bands from an uncharacterized human DNA
Definition from:
Human Molecular Genetics 2, Tom Strachan & Andrew
P. Read
Amplicon
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An amplicon is a small piece
of DNA that has been amplified by PCR
Definition from:
http://www.bioexchange.com/news/news_page.cfm?id=8306
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The product of PCR or LCR; a piece of DNA
that has been synthesized using amplification techniques
Definition from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-A/amplicon.html
Amplification
See DNA amplification
Annealing
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Annealing is a process where the forward and reverse primers anneal to
separated DNA template strand.
Definition from:http://www.ppsk.usm.my/lecturers/mravi/PDF_FIles/Group_3_2002.pdf
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Annealing is a bimolecular process, but there are competing processes in
which both intrastrand and interstrand base pairs can form, ultimately
leading to the stable duplex
Definition from:
http://www.biochem.arizona.edu/classes/bioc462/462a/NOTES/
Nucleic_Acids/nucacid_structure.html
Asymmetric PCR
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Asymmetric PCR is used to preferentially amplify one strand of the
original DNA more than the other. It finds use in some types of sequencing
and hybridization probing where having only one of the two complementary
stands is ideal. PCR is carried out as usual, but with a great excess of
the primers for the chosen strand. Due to the slow (arithmetic)
amplification later in the reaction after the limiting primer has been
used up, extra cycles of PCR are required
Definition from:
Polymerase chain reaction, Wikipedia, The Free Encyclopedia
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